Practical Fragments

Differential scanning fluorimetry (DSF), also known as a thermal shift assay, is one of the most common techniques for fragment screening ac...

Last week we described a fragment-derived covalent probe that targets the four closely related TEAD transcription factors, which are part o...

1.2 million deaths. If you did not read the title of this post carefully you may assume this statistic refers to COVID-19. In fact, it is th...

Many of the posts on Practical Fragments – and indeed much of what appears in the literature – describe successes. This is obvious in the ...

The Mycobacterium genus of bacteria contains more than 190 species, including the pathogens that cause leprosy and tuberculosis. Mycobacte...

Epigenetics is a topic we’ve covered frequently at Practical Fragments . Much attention has been focused on bromodomains , which recognize ...

The 2017 International Symposium on Fragment Based Lead Discovery (pdf here ) was held in Shanghai, China last week. I was fortunate to be ...

Ligandability refers to the ability to find small-molecule leads against a target. A protein might be ligandable but not druggable if, for...

Native electrospray ionization mass spectrometry (ESI-MS) is one of the less-commonly used fragment finding methods . The technique relies ...

Protein-protein disruptions are notoriously difficult because the interfaces between proteins tend to be large and flat, with few of the de...

One of the less commonly used fragment-finding methods is microscale thermophoresis ( MST ). This measures the movement of proteins in a t...

In the Northern Hemisphere the winter solstice has passed but the days are still short, and 2015 is hurtling into history. As we did in 201...