tag:blogger.com,1999:blog-1136153439451224584.post8302231162415633103..comments2024-03-27T06:45:59.174-07:00Comments on Practical Fragments: Poll: fragment screening methodsDr. Teddy Zhttp://www.blogger.com/profile/07288045760981372367noreply@blogger.comBlogger4125tag:blogger.com,1999:blog-1136153439451224584.post-1722198628171111222011-09-09T07:23:02.002-07:002011-09-09T07:23:02.002-07:00and another rather easy thing: higher well volume ...and another rather easy thing: higher well volume (i.e. 40 instead of 20 uL)Michele Snoreply@blogger.comtag:blogger.com,1999:blog-1136153439451224584.post-55732944668370601622011-09-09T07:18:52.025-07:002011-09-09T07:18:52.025-07:00maybe that's obvious, but did you test differe...maybe that's obvious, but did you test different buffers?Michele Snoreply@blogger.comtag:blogger.com,1999:blog-1136153439451224584.post-45655495046481006332011-09-09T06:29:51.698-07:002011-09-09T06:29:51.698-07:00Better S/N for thermal shift assays (DSF):
Screen...Better S/N for thermal shift assays (DSF):<br /><br />Screen protein concentrations vs SYPRO Orange concentration.<br />Accept that some compounds just screw the assay.<br />Purer sample.<br />Apply Andrew Yeh's methods for background subtraction: http://www.ncbi.nlm.nih.gov/pubmed/16552147<br />Acquire a machine for differential static light scattering (DSLS).<br /><br />Ordered by easiest to hardest/most cumbersome.Morten Grøftehaugehttps://www.blogger.com/profile/05252471246488393284noreply@blogger.comtag:blogger.com,1999:blog-1136153439451224584.post-86113991470371128452011-09-08T07:34:50.350-07:002011-09-08T07:34:50.350-07:00Anyone have any hints on how to increase S/N for t...Anyone have any hints on how to increase S/N for thermal shift assays?Anonymousnoreply@blogger.com