tag:blogger.com,1999:blog-1136153439451224584.post6744643373500804568..comments2024-03-27T06:45:59.174-07:00Comments on Practical Fragments: Poll results: what structural information is needed to optimize fragmentsDr. Teddy Zhttp://www.blogger.com/profile/07288045760981372367noreply@blogger.comBlogger1125tag:blogger.com,1999:blog-1136153439451224584.post-40700930245485536062017-06-07T18:20:00.137-07:002017-06-07T18:20:00.137-07:00How about before hit optimization?
This might hav...How about before hit optimization?<br /><br />This might have been polled, but I'd like to see how people stick to the so-called "rule-of-three" when assembling fragments for their library.<br /><br />Practically, would you also start with fragments with sub-optimal aqueous solubility? This may depend on the size of the fragment, but I found for smaller fragments (MW ~ 200 or less), a solubility of 1 mM (with 1% DMSO in PBS) is usually not sufficient for functional assays since they often bind very weakly to the target.Anonymoushttps://www.blogger.com/profile/18006385649846576237noreply@blogger.com