tag:blogger.com,1999:blog-1136153439451224584.post7804414738587271365..comments2024-03-27T06:45:59.174-07:00Comments on Practical Fragments: LO-MS...Coming of Age.Dr. Teddy Zhttp://www.blogger.com/profile/07288045760981372367noreply@blogger.comBlogger1125tag:blogger.com,1999:blog-1136153439451224584.post-9005818677694719372015-10-21T21:35:44.456-07:002015-10-21T21:35:44.456-07:00I guess I was a little less sanguine about the tec...I guess I was a little less sanguine about the technique after reading this paper. The weakest fragment validated by ITC was still quite strong, with a dissociation constant of 37 micromolar.<br /><br />Worse, depending on the assay conditions, dissociation constants measured for the same ligand could vary by more than an order of magnitude. If experts in the technique find this much variation with a well-characterized protein such as carbonic anhydrase, it may prove troublesome in less experienced hands for more difficult targets.Dan Erlansonhttps://www.blogger.com/profile/07927082337051189270noreply@blogger.com